In this experiment , the cultured virus was detected by pcr amplification . moreover , 3 natural sheep cases , 16 sheep cases and 16 goat cases at random were diagnosed . the results showed 3 natural sheep cases were spa , 2 of 16 sheep were positive and others were negative , 16 goats were negative 本試驗還應用pcr技術,對培養的病毒進行檢測;并對自然感染spa的3例綿羊、隨機采取的16例山羊和16例綿羊進行了診斷,結果表明:自然感染的3例綿羊確實患有spa ,為陽性;隨機采取的16例綿羊中有2例為陽性,其余14例為陰性; 16例山羊均為陰性,未感染spa 。